ABSTRACT
Objective To build a rat model of intrauterine adhesion (IUA) by whole layer removal of endometrium in order to provide a suitable animal model for the treatment and experimental study of endometrial lesions and IUA.Methods According to different scopes of endometrial excision,105 female SD rats were divided into group A (2.5 cm × 0.5 cm),group B (2.5 cm × 0.25 cm) and group C (sham operation group) with 35 rats in each group.Uterine tissues of rats were collected 3,7,15 and 30 days after surgery.The ratio of uterine cavity narrow and congestion was recorded,the number of glands in the endometrium and the ratio of endometrial fibrosis area was counted,microvascular density (MVD) was measured,and pregnancy rate of rats in each group were observed to compare the two excision methods.Results The ratio of fibrosis area in group A and group B (0.892 ± 0.068,0.562 ± 0.027) were higher than that in group C (0.374 ± 0.074).Uterus patency scores in group A and group B (1.87 ± 0.16,1.20 ± 0.17) were both significantly higher than that in group C (0.68 ±0.098).The differences were statistically significant (P ＜ 0.001).The numbers of glands in the endometrium 30 days after surgery in group A,B and C were 7.35 ± 2.19,14.5 ± 2.43 and 15.56 ± 2.63,respectively,significantly less in group A than that in group B and C (both P＜0.001),while there was no statistical difference between group B and C (P =0.054).The MVD in group A,B and C were 16.06 ± 2.11,24.6 ± 2.34 and 26.37 ± 5.09,respectively,significantly less in group A than that in group B and C (both P＜0.001),while there was no statistical differences between group B and C (P=0.303).The rates of pregnancy in group A,B and C were 20％,33.3％ and 100％,respectively,obviously lower in group A than that in group B and C (P＜0.05).Conclusions The method of whole layer removal of endometriun had a high success rate in establishing a stable and effective rat model of IUA.
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate value of spectral karyotyping (SKY) in the detection of chromosomal abnormalities.</p><p><b>METHODS</b>A total of 17 metaphase chromosome samples were investigated by SKY, including 10 normal and 5 balanced translocation samples of peripheral blood lymphocytes, one der(Y) sample of peripheral blood lymphocytes and one marker chromosome sample of amniotic fluid cells. The results were compared with those of G-banding diagnosis.</p><p><b>RESULTS</b>Ten normal and 5 balanced translocation samples were diagnosed successfully by SKY in accordance with the results of G-banding; furthermore, SKY analysis revealed that the der(Y) fragment originated from p-arm of chromosome 21 while the marker chromosome originated from chromosome 5.</p><p><b>CONCLUSION</b>SKY is a very sensitive and specific whole genome analysis tool for chromosomal abnormality diagnosis, and exceedingly valuable in the diagnosis on complex chromosomal abnormalities that can not be determined by G-banding.</p>